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And a single transfection and selection step for cell lines.

This new method for creating stable AAV2 producer cell lines overcomes the major drawbacks of the previous method: it requires only a one-step cloning process , and a single transfection and selection step for cell lines, which required the circular AAV vector-containing plasmid DNA, carry a gene of interest a gene of interest in host cells.

With improvements in cancer treatment in the last two decades, children are living longer with soft tissue sarcomas. Several studies have reported that these children have an increased risk of second cancer, but estimates of risk parameters have very, very diverse and range from 3 to 13 times the risk in the general population. In addition, the small size of many previous studies , few risk by type of soft tissue sarcoma evaluated or have estimated risks for specific second cancers.